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J. Huggett · SciRah

Researcher profile · Semantic Scholar

J. Huggett

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J. Huggett — researcher profile on SciRah

h-index: 49
Works: 169
Citations: 26K

Most-cited papers

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.
J. Huggett, C. Foy, V. Beneš, K. Emslie +11 more
2013Clinical Chemistry803 citations Open access
Semantic Scholar
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020.
J. Huggett, A. Whale, W. De Spiegelaere, Wim Trypsteen +29 more
2020Clinical Chemistry475 citations Open access
Semantic Scholar
Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs
J. Phelan, D. O'Sullivan, D. Machado, J. Ramos +8 more
2019Genome Medicine448 citations Open access
Semantic Scholar
Considerations for digital PCR as an accurate molecular diagnostic tool.
J. Huggett, S. Cowen, C. Foy
2015Clinical Chemistry416 citations
Semantic Scholar
Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification
Alison S. Devonshire, A. Whale, A. Gutteridge, Gerwyn M. Jones +3 more
2014Analytical and Bioanalytical Chemistry313 citations Open access
Semantic Scholar
The need for transparency and good practices in the qPCR literature
S. Bustin, V. Beneš, J. Garson, J. Hellemans +80 more
2013Nature Methods283 citations Open access
Semantic Scholar
qPCR primer design revisited
S. Bustin, J. Huggett
2017Biomolecular Detection and Quantification279 citations Open access
Semantic Scholar
Evaluation of Digital PCR for Absolute RNA Quantification
Rebecca Sanders, D. Mason, C. Foy, J. Huggett
2013PLoS ONE175 citations Open access
Semantic Scholar
Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples
A. Whale, S. Cowen, C. Foy, J. Huggett
2013PLoS ONE158 citations Open access
Semantic Scholar
Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA
D. Gale, Andrew R. J. Lawson, K. Howarth, M. Madi +22 more
2018PLoS ONE143 citations Open access
Semantic Scholar
Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.
G. Nixon, J. Garson, P. Grant, E. Nastouli +2 more
2014Analytical Chemistry133 citations
Semantic Scholar
Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study
R. Doyle, D. O'Sullivan, Sean Aller, Sebastian Bruchmann +17 more
2019bioRxiv122 citations Open access
Semantic Scholar
Digital PCR as a novel technology and its potential implications for molecular diagnostics.
J. Huggett, A. Whale
2013Clinical Chemistry112 citations Open access
Semantic Scholar
Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples
Rebecca Sanders, D. Mason, C. Foy, J. Huggett
2014Analytical and Bioanalytical Chemistry100 citations Open access
Semantic Scholar
Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.
Alison S. Devonshire, I. Honeyborne, A. Gutteridge, A. Whale +6 more
2015Analytical Chemistry92 citations
Semantic Scholar
Minimum information necessary for quantitative real-time PCR experiments.
Gemma L. Johnson, A. Abdel Nour, T. Nolan, J. Huggett +1 more
2014Methods in molecular biology83 citations Open access
Semantic Scholar
Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine.
A. Whale, Gerwyn M. Jones, J. Pavšič, T. Dreo +23 more
2018Clinical Chemistry75 citations Open access
Semantic Scholar
RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia.
M. Alikian, A. Whale, S. Akiki, K. Piechocki +9 more
2016Clinical Chemistry73 citations
Semantic Scholar
Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis.
Alison S. Devonshire, Rebecca Sanders, T. Wilkes, Martin S. Taylor +2 more
2013Methods72 citations
Semantic Scholar
STROBE-metagenomics: a STROBE extension statement to guide the reporting of metagenomics studies
T. Bharucha, C. Oeser, F. Balloux, Julianne R. Brown +23 more
2020Lancet. Infectious Diseases (Print)70 citations Open access
Semantic Scholar
International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant.
A. Whale, Alison S. Devonshire, G. Karlin-Neumann, John F. Regan +30 more
2017Analytical Chemistry67 citations Open access
Semantic Scholar
The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs
J. Phelan, D. O'Sullivan, D. Machado, J. Ramos +8 more
2016Genome Medicine62 citations Open access
Semantic Scholar
The dangers of using Cq to quantify nucleic acid in biological samples; a lesson from COVID-19.
D. Evans, S. Cowen, M. Kammel, D. O'Sullivan +16 more
2021Clinical Chemistry60 citations Open access
Semantic Scholar
Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
J. Huggett, V. Beneš, S. Bustin, J. Garson +13 more
2020Clinical Chemistry59 citations Open access
Semantic Scholar
Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis
J. Garson, L. Usher, A. Al-Chalabi, J. Huggett +2 more
2019Acta Neuropathologica Communications56 citations Open access
Semantic Scholar
Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples
A. Whale, C. Bushell, P. Grant, S. Cowen +8 more
2015Journal of Clinical Microbiology55 citations Open access
Semantic Scholar
The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis
Alison S. Devonshire, D. O'Sullivan, I. Honeyborne, Gerwyn M. Jones +27 more
2016BMC Infectious Diseases50 citations Open access
Semantic Scholar
Detection of Mycobacterium tuberculosis complex DNA in CD34-positive peripheral blood mononuclear cells of asymptomatic tuberculosis contacts: an observational study
M. Belay, B. Tulu, S. Younis, David A. Jolliffe +25 more
2021The Lancet Microbe49 citations Open access
Semantic Scholar
Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis
N. Redshaw, J. Huggett, Martin S. Taylor, C. Foy +1 more
2014BMC Genomics47 citations Open access
Semantic Scholar
Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses
J. Pavšič, Alison S. Devonshire, H. Parkes, H. Schimmel +9 more
2014Journal of Clinical Microbiology44 citations Open access
Semantic Scholar
Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments
J. Huggett, C. Foy, V. Beneš, K. Emslie +11 more
201344 citations
Semantic Scholar
Monkeypox: another test for PCR
J. Huggett, David French, D. O'Sullivan, Jacob Moran-Gilad +1 more
2022Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin41 citations Open access
Semantic Scholar
International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.
Hee-Bong Yoo, Sang-Ryoul Park, Lian-Hua Dong, Jing Wang +20 more
2016Analytical Chemistry41 citations
Semantic Scholar
RNA reference materials with defined viral RNA loads of SARS-CoV-2—A useful tool towards a better PCR assay harmonization
L. Vierbaum, Nathalie Wojtalewicz, H. Grunert, Vanessa Lindig +23 more
2022PLoS ONE39 citations Open access
Semantic Scholar
Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR
Eloise J. Busby, A. Whale, R. Ferns, P. Grant +6 more
2017Scientific Reports39 citations Open access
Semantic Scholar
An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities
D. O'Sullivan, R. Doyle, Sasithon Temisak, N. Redshaw +24 more
2021Scientific Reports38 citations Open access
Semantic Scholar
Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.
M. Alikian, P. Ellery, M. Forbes, G. Gerrard +9 more
2016Journal of Molecular Diagnostics38 citations Open access
Semantic Scholar
Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.
Daniel Marks, Catherine Hyams, C. Koo, M. Pavlou +9 more
2015QJM : monthly journal of the Association of Physicians36 citations
Semantic Scholar
Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA
J. Pavšič, Alison S. Devonshire, A. Blejec, C. Foy +11 more
2017Analytical and Bioanalytical Chemistry33 citations Open access
Semantic Scholar
Improving the standardization of mRNA measurement by RT-qPCR
Rebecca Sanders, Stephen A. Bustin, J. Huggett, D. Mason
2018Biomolecular Detection and Quantification29 citations Open access
Semantic Scholar
A phantom-based evaluation of three commercially available patient organ shields for computed tomography X-ray examinations in diagnostic radiology.
J. Huggett, W. Mukonoweshuro, R. Loader
2013Radiation Protection Dosimetry28 citations
Semantic Scholar
Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics
A. Whale, E. K. von der Heide, Max Kohlenberg, A. Brinckmann +11 more
2021Methods26 citations Open access
Semantic Scholar
An assessment of the reproducibility of reverse transcription digital PCR quantification of HIV-1.
S. Falak, R. Macdonald, Eloise J. Busby, D. O'Sullivan +7 more
2021Methods25 citations Open access
Semantic Scholar
Considerations for the development and application of control materials to improve metagenomic microbial community profiling
J. Huggett, T. Laver, Sasithon Tamisak, G. Nixon +4 more
2013Accreditation and Quality Assurance25 citations Open access
Semantic Scholar
Assessing the Accuracy of Quantitative Molecular Microbial Profiling
D. O'Sullivan, T. Laver, Sasithon Temisak, N. Redshaw +4 more
2014International Journal of Molecular Sciences24 citations Open access
Semantic Scholar
qPCR, dPCR, NGS – A journey
J. Huggett, J. O'Grady, Stephen A. Bustin
2015Biomolecular Detection and Quantification23 citations Open access
Semantic Scholar
Distribution of Tetrodotoxin in Pacific Oysters (Crassostrea gigas)
Monika Dhanji-Rapkova, A. Turner, C. Baker-Austin, J. Huggett +1 more
2021Marine Drugs22 citations Open access
Semantic Scholar
Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes
Julianne R. Brown, D. O'Sullivan, Divya Shah, Laura Atkinson +5 more
2021Journal of Virological Methods22 citations Open access
Semantic Scholar
Systematic review with meta-analysis of diagnostic test accuracy for COVID-19 by mass spectrometry
M. Spick, H. Lewis, M. Wilde, C. Hopley +2 more
2021Metabolism: Clinical and Experimental21 citations Open access
Semantic Scholar
Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements
M. Milavec, M. Cleveland, Young-Kyung Bae, R. Wielgosz +2 more
2021Analytical and Bioanalytical Chemistry19 citations Open access
Semantic Scholar

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