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Tomokazu Shirai · SciRah

Researcher profile · Semantic Scholar

Tomokazu Shirai

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Tomokazu Shirai — researcher profile on SciRah

h-index: 23
Works: 78
Citations: 1,487

Most-cited papers

Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives.
Shuhei Noda, Tomokazu Shirai, Sachiko Oyama, A. Kondo
2016Metabolic Engineering118 citations
Semantic Scholar
Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol
Hiroshi Teramura, K. Sasaki, T. Oshima, F. Matsuda +9 more
2016Biotechnology for Biofuels85 citations Open access
Semantic Scholar
Capillary electrophoresis-mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803.
T. Osanai, A. Oikawa, Tomokazu Shirai, A. Kuwahara +6 more
2014Environmental Microbiology80 citations
Semantic Scholar
Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum.
Hiroki Sato, K. Orishimo, Tomokazu Shirai, T. Hirasawa +3 more
2008Journal of Bioscience and Bioengineering79 citations
Semantic Scholar
Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium
T. Osanai, Tomokazu Shirai, H. Iijima, Y. Nakaya +3 more
2015Frontiers in Microbiology74 citations Open access
Semantic Scholar
Construction of a Model Culture System of Human Colonic Microbiota to Detect Decreased Lachnospiraceae Abundance and Butyrogenesis in the Feces of Ulcerative Colitis Patients.
K. Sasaki, J. Inoue, D. Sasaki, Namiko Hoshi +5 more
2019Biotechnology Journal66 citations Open access
Semantic Scholar
Comparative study of flux redistribution of metabolic pathway in glutamate production by two coryneform bacteria.
Tomokazu Shirai, Akinori Nakato, N. Izutani, K. Nagahisa +6 more
2005Metabolic Engineering61 citations
Semantic Scholar
Investigating the effects of perturbations to pgi and eno gene expression on central carbon metabolism in Escherichia coli using 13 C metabolic flux analysis
Yukihiko Usui, T. Hirasawa, C. Furusawa, Tomokazu Shirai +3 more
2012Microbial Cell Factories55 citations Open access
Semantic Scholar
Group 2 Sigma Factor SigB of Corynebacterium glutamicum Positively Regulates Glucose Metabolism under Conditions of Oxygen Deprivation
S. Ehira, Tomokazu Shirai, H. Teramoto, M. Inui +1 more
2008Applied and Environmental Microbiology55 citations Open access
Semantic Scholar
Engineering a synthetic pathway for maleate in Escherichia coli
Shuhei Noda, Tomokazu Shirai, Yutaro Mori, Sachiko Oyama +1 more
2017Nature Communications43 citations Open access
Semantic Scholar
Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant
Yutaro Mori, Shuhei Noda, Tomokazu Shirai, A. Kondo
2020Nature Communications41 citations Open access
Semantic Scholar
Identification of a Gene Encoding a Transporter Essential for Utilization of C4 Dicarboxylates in Corynebacterium glutamicum
H. Teramoto, Tomokazu Shirai, M. Inui, H. Yukawa
2008Applied and Environmental Microbiology36 citations Open access
Semantic Scholar
Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum.
Shunsuke Kobayashi, H. Kawaguchi, Tomokazu Shirai, Kazuaki Ninomiya +3 more
2020ACS Synthetic Biology33 citations
Semantic Scholar
Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration.
K. Sasaki, M. Okamoto, Tomokazu Shirai, Y. Tsuge +6 more
2016Bioresource Technology33 citations
Semantic Scholar
Changes in Lignin and Polysaccharide Components in 13 Cultivars of Rice Straw following Dilute Acid Pretreatment as Studied by Solution-State 2D 1H-13C NMR
Hiroshi Teramura, K. Sasaki, T. Oshima, S. Aikawa +8 more
2015PLoS ONE31 citations Open access
Semantic Scholar
Evaluating 13C enrichment data of free amino acids for precise metabolic flux analysis
Eiji Mori, C. Furusawa, Shuichi Kajihata, Tomokazu Shirai +1 more
2011Biotechnology Journal31 citations
Semantic Scholar
Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria
Sakiko Ueda, Yuhki Kawamura, H. Iijima, Mitsuharu Nakajima +5 more
2016Scientific Reports30 citations Open access
Semantic Scholar
Alteration of cyanobacterial sugar and amino acid metabolism by overexpression hik8, encoding a KaiC-associated histidine kinase.
T. Osanai, Tomokazu Shirai, H. Iijima, A. Kuwahara +3 more
2015Environmental Microbiology30 citations
Semantic Scholar
Changes in primary metabolism under light and dark conditions in response to overproduction of a response regulator RpaA in the unicellular cyanobacterium Synechocystis sp. PCC 6803
H. Iijima, Tomokazu Shirai, M. Okamoto, A. Kondo +2 more
2015Frontiers in Microbiology30 citations Open access
Semantic Scholar
Cocktail δ-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae.
Hiroko Kato, F. Matsuda, Ryosuke Yamada, Ken Nagata +3 more
2013Journal of Bioscience and Bioengineering30 citations
Semantic Scholar
Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
Shuhei Noda, Yutaro Mori, Sachiko Oyama, A. Kondo +2 more
2019Microbial Cell Factories29 citations Open access
Semantic Scholar
Metabolic engineering of isopropyl alcohol‐producing Escherichia coli strains with 13C‐metabolic flux analysis
Nobuyuki Okahashi, F. Matsuda, K. Yoshikawa, Tomokazu Shirai +3 more
2017Biotechnology and Bioengineering27 citations
Semantic Scholar
Metabolic and enzymatic engineering approach for the production of 2-phenylethanol in engineered Escherichia coli.
Shuhei Noda, Yutaro Mori, Yuki Ogawa, Ryosuke Fujiwara +3 more
2024Bioresource Technology25 citations Open access
Semantic Scholar
Four-carbon dicarboxylic acid production through the reductive branch of the open cyanobacterial tricarboxylic acid cycle in Synechocystis sp. PCC 6803.
H. Iijima, A. Watanabe, Haruna Sukigara, Kaori Iwazumi +3 more
2021Metabolic Engineering22 citations Open access
Semantic Scholar
Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model
Tomokazu Shirai, T. Osanai, A. Kondo
2016Microbial Cell Factories22 citations Open access
Semantic Scholar
Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content
K. Sasaki, M. Okamoto, Tomokazu Shirai, Y. Tsuge +8 more
2015Biotechnology for Biofuels22 citations Open access
Semantic Scholar
Precise metabolic flux analysis of coryneform bacteria by gas chromatography-mass spectrometry and verification by nuclear magnetic resonance.
Tomokazu Shirai, Kunihiro Matsuzaki, M. Kuzumoto, K. Nagahisa +3 more
2006Journal of Bioscience and Bioengineering20 citations
Semantic Scholar
Dark accumulation of downstream glycolytic intermediates initiates robust photosynthesis in cyanobacteria.
Kenya Tanaka, Tomokazu Shirai, C. Vavricka, Mami Matsuda +2 more
2022Plant Physiology19 citations Open access
Semantic Scholar
Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis
Nunthaphan Vikromvarasiri, Tomokazu Shirai, A. Kondo
2021Microbial Cell Factories19 citations Open access
Semantic Scholar
Reprogramming Escherichia coli pyruvate-forming reaction towards chorismate derivatives production.
Shuhei Noda, Yutaro Mori, Ryosuke Fujiwara, Tomokazu Shirai +2 more
2021Metabolic Engineering19 citations Open access
Semantic Scholar
Molecular mechanisms and metabolic engineering of glutamate overproduction in Corynebacterium glutamicum.
T. Hirasawa, Jongpill Kim, Tomokazu Shirai, C. Furusawa +1 more
2012Sub-cellular biochemistry19 citations
Semantic Scholar
NHC-Mediated Radical Acylation Catalyzed by Thiamine- and Flavin-Dependent Enzymes
Shunsuke Kato, Shuto Fujisawa, Y. Adachi, Mitsuhiro Bandai +4 more
2025Journal of the American Chemical Society17 citations Open access
Semantic Scholar
Optimal Ratio of Carbon Flux between Glycolysis and the Pentose Phosphate Pathway for Amino Acid Accumulation in Corynebacterium glutamicum.
K. Murai, D. Sasaki, Shunsuke Kobayashi, A. Yamaguchi +5 more
2020ACS Synthetic Biology16 citations
Semantic Scholar
Designing artificial metabolic pathways, construction of target enzymes, and analysis of their function.
Yutaro Mori, Tomokazu Shirai
2018Current Opinion in Biotechnology16 citations
Semantic Scholar
Metabolomics-based analysis revealing the alteration of primary carbon metabolism by the genetic manipulation of a hydrogenase HoxH in Synechocystis sp. PCC 6803
H. Iijima, Tomokazu Shirai, M. Okamoto, F. Pinto +5 more
201616 citations Open access
Semantic Scholar
Aromatic secondary metabolite production from glycerol was enhanced by amino acid addition in Pichia pastoris
Ryota Kumokita, T. Yoshida, Tomokazu Shirai, Akihiko Kondo +1 more
2023Applied Microbiology and Biotechnology14 citations Open access
Semantic Scholar
Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis
Nunthaphan Vikromvarasiri, Shuhei Noda, Tomokazu Shirai, Akihiko Kondo
2023Journal of Biological Engineering11 citations Open access
Semantic Scholar
Engineered Nanogel Particles Enhance the Photoautotrophic Biosynthesis of Polyhydroxyalkanoate in Marine Photosynthetic Bacteria
Pisanee Srisawat, Mieko Higuchi-Takeuchi, Ryutaro Honda, Tomokazu Shirai +3 more
2022ACS Sustainable Chemistry & Engineering11 citations Open access
Semantic Scholar
Biotechnology and Bioengineering
H. Iijima, A. Watanabe, Haruna Sukigara, Tomokazu Shirai +2 more
202010 citations
Semantic Scholar
Efficient 3-Hydroxybutyrate Production by Quiescent Escherichia coli Microbial Cell Factories is Facilitated by Indole-Induced Proteomic and Metabolomic Changes.
Nicholas M. Thomson, Tomokazu Shirai, M. Chiapello, A. Kondo +4 more
2018Biotechnology Journal10 citations Open access
Semantic Scholar
Evaluation of nitrogen fixation in the marine purple photosynthetic bacterium Rhodovulum sulfidophilum under autotrophic and heterotrophic conditions
Miki Suzuki, Tomokazu Shirai, Shamitha Rao Morey-Yagi, Akihiko Kondo +1 more
2025Scientific Reports7 citations Open access
Semantic Scholar
Examination of the effect of HOG1 deletion on glucose fermentation in Saccharomyces cerevisiae.
Nunthaphan Vikromvarasiri, Ryosuke Mitsui, Takashi Hirasawa, Akihiko Kondo +1 more
2025Bioresource Technology7 citations Open access
Semantic Scholar
Production of (R)-citramalate by engineered Saccharomyces cerevisiae
Ryosuke Mitsui, Akihiko Kondo, Tomokazu Shirai
2024Metabolic Engineering Communications7 citations Open access
Semantic Scholar
G6P-capturing molecules in the periplasm of Escherichia coli accelerate the shikimate pathway.
Ryosuke Fujiwara, M. Nakano, Yuuki Hirata, Chisako Otomo +8 more
2022Metabolic Engineering7 citations Open access
Semantic Scholar
History-Driven Genetic Modification Design Technique Using a Domain-Specific Lexical Model for the Acceleration of DBTL Cycles for Microbial Cell Factories.
Shiori Nakazawa, Osamu Imaichi, Takahisa Kogure, Takeshi Kubota +6 more
2021ACS Synthetic Biology7 citations Open access
Semantic Scholar
Evaluation of control mechanisms for Saccharomyces cerevisiae central metabolic reactions using metabolome data of eight single-gene deletion mutants
Tomokazu Shirai, F. Matsuda, M. Okamoto, A. Kondo
2013Applied Microbiology and Biotechnology7 citations
Semantic Scholar
Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis
Tomokazu Shirai, Koki Fujimura, C. Furusawa, K. Nagahisa +2 more
2007Microbial Cell Factories7 citations Open access
Semantic Scholar
Styrene Production in Genetically Engineered Escherichia coli in a Two-Phase Culture
Shuhei Noda, Ryosuke Fujiwara, Yutaro Mori, Mayumi Dainin +2 more
2024BioTech5 citations Open access
Semantic Scholar
13C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae.
Taiji Yuzawa, Tomokazu Shirai, Ryoko Orishimo, Kazuki Kawai +2 more
2021Journal of General and Applied Microbiology5 citations Open access
Semantic Scholar
Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L-Tyrosine Over-Producing Saccharomyces cerevisiae
Shuhei Noda, Tomokazu Shirai, K. Mochida, F. Matsuda +3 more
2015PLoS ONE5 citations Open access
Semantic Scholar

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A. Kondo×27
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A. Kondo — researcher profile on SciRah
Shuhei Noda×10
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Shuhei Noda — researcher profile on SciRah
M. Okamoto×10
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M. Okamoto — researcher profile on SciRah
T. Osanai×9
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F. Matsuda — researcher profile on SciRah

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H. Iijima — researcher profile on SciRah

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H. Shimizu — researcher profile on SciRah

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Yutaro Mori — researcher profile on SciRah

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K. Sasaki — researcher profile on SciRah

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M. Hirai — researcher profile on SciRah

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C. Furusawa — researcher profile on SciRah

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T. Hasunuma — researcher profile on SciRah