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E. M. Gerhardt · SciRah

Researcher profile · Semantic Scholar

E. M. Gerhardt

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E. M. Gerhardt — researcher profile on SciRah

h-index: 13
Works: 41
Citations: 523

Most-cited papers

Magnetic Bead-Based Immunoassay Allows Rapid, Inexpensive, and Quantitative Detection of Human SARS-CoV-2 Antibodies
L. Huergo, K. Selim, M. Conzentino, E. M. Gerhardt +23 more
2021ACS Sensors73 citations Open access
Semantic Scholar
The Bacterial signal transduction protein GlnB regulates the committed step in fatty acid biosynthesis by acting as a dissociable regulatory subunit of acetyl‐CoA carboxylase
E. M. Gerhardt, Thiago E. Rodrigues, Marcelo Muller-Santos, F. O. Pedrosa +3 more
2015Molecular Microbiology66 citations Open access
Semantic Scholar
Interaction of the Nitrogen Regulatory Protein GlnB (PII) with Biotin Carboxyl Carrier Protein (BCCP) Controls Acetyl-CoA Levels in the Cyanobacterium Synechocystis sp. PCC 6803
Waldemar Hauf, K. Schmid, E. M. Gerhardt, L. Huergo +1 more
2016Frontiers in Microbiology52 citations Open access
Semantic Scholar
Crystal structure of the GlnZ-DraG complex reveals a different form of PII-target interaction
C. Rajendran, E. M. Gerhardt, S. Bjelić, Antonietta Gasperina +8 more
2011Proceedings of the National Academy of Sciences of the United States of America44 citations Open access
Semantic Scholar
Search for novel targets of the PII signal transduction protein in Bacteria identifies the BCCP component of acetyl‐CoA carboxylase as a PII binding partner
Thiago E. Rodrigues, E. M. Gerhardt, M. A. S. Oliveira, L. Chubatsu +5 more
2014Molecular Microbiology35 citations Open access
Semantic Scholar
Dynamics of the Escherichia coli proteome in response to nitrogen starvation and entry into the stationary phase.
H. S. Sanchuki, Fernanda Gravina, Thiago E. Rodrigues, E. M. Gerhardt +6 more
2017Biochimica et Biophysica Acta - Proteins and Proteomics26 citations
Semantic Scholar
Heat stability of Proteobacterial PII protein facilitate purification using a single chromatography step.
V. Moure, Guilherme Razzera, L. Araújo, M. A. S. Oliveira +7 more
2012Protein Expression and Purification24 citations Open access
Semantic Scholar
NAD+ biosynthesis in bacteria is controlled by global carbon/nitrogen levels via PII signaling
A. Santos, E. M. Gerhardt, Erick Parize, F. O. Pedrosa +8 more
2020Journal of Biological Chemistry20 citations Open access
Semantic Scholar
The Nitrogenase Regulatory Enzyme Dinitrogenase Reductase ADP-Ribosyltransferase (DraT) Is Activated by Direct Interaction with the Signal Transduction Protein GlnB
V. Moure, Karamatullah Danyal, Zhi-Yong Yang, S. Wendroth +7 more
2012Journal of Bacteriology20 citations Open access
Semantic Scholar
Influence of the ADP/ATP ratio, 2-oxoglutarate and divalent ions on Azospirillum brasilense PII protein signalling.
E. M. Gerhardt, L. Araújo, R. Ribeiro, L. Chubatsu +6 more
2012Microbiology18 citations Open access
Semantic Scholar
In vitro interaction between the ammonium transport protein AmtB and partially uridylylated forms of the P(II) protein GlnZ.
Thiago E. Rodrigues, Victor E. P. Souza, R. Monteiro, E. M. Gerhardt +5 more
2011Biochimica et Biophysica Acta17 citations Open access
Semantic Scholar
The protein-protein interaction network of the Escherichia coli EIIANtr regulatory protein reveals a role in cell motility and metabolic control.
Fernanda Gravina, F. L. Degaut, E. M. Gerhardt, F. Pedrosa +3 more
2021Research in Microbiology14 citations Open access
Semantic Scholar
The Protein-Protein Interaction Network Reveals a Novel Role of the Signal Transduction Protein PII in the Control of c-di-GMP Homeostasis in Azospirillum brasilense
E. M. Gerhardt, Erick Parize, Fernanda Gravina, Flávia L. D. Pontes +13 more
2020mSystems14 citations Open access
Semantic Scholar
Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes.
Fernanda Gravina, H. S. Sanchuki, Thiago E. Rodrigues, E. M. Gerhardt +5 more
2018Journal of Proteomics13 citations
Semantic Scholar
2‐Oxoglutarate levels control adenosine nucleotide binding by Herbaspirillum seropedicae PII proteins
M. A. S. Oliveira, E. M. Gerhardt, L. Huergo, E. M. Souza +2 more
2015The FEBS Journal11 citations Open access
Semantic Scholar
The NADP-dependent malic enzyme MaeB is a central metabolic hub controlled by the acetyl-CoA to CoASH ratio.
L. Huergo, Gillize A. T. Araújo, A. Santos, E. M. Gerhardt +3 more
2020Biochimica et Biophysica Acta - Proteins and Proteomics10 citations Open access
Semantic Scholar
Production, purification, and characterization of a novel serine‐esterase from Aspergillus westerdijkiae
F. F. Castro, A. Pinheiro, E. M. Gerhardt, M. A. S. Oliveira +1 more
2018Journal of Basic Microbiology10 citations
Semantic Scholar
Kinetics and structural features of dimeric glutamine-dependent bacterial NAD+ synthetases suggest evolutionary adaptation to available metabolites
A. Santos, E. M. Gerhardt, V. Moure, F. O. Pedrosa +4 more
2018Journal of Biological Chemistry9 citations Open access
Semantic Scholar
Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense.
Fernanda Ghenov, E. M. Gerhardt, L. Huergo, F. O. Pedrosa +2 more
2021Brazilian journal of biology = Revista brasleira de biologia7 citations Open access
Semantic Scholar
Unlocking nitrogen regulation: structural insights into the NifL‐NifA complex and prospects for engineered diazotrophs
E. M. Gerhardt, Khaled A. Selim
2025The FEBS Journal6 citations Open access
Semantic Scholar
The PII protein interacts with the Amt ammonium transport and modulates nitrate/nitrite assimilation in mycobacteria
Delfina Ensinck, E. M. Gerhardt, Lara Rollan, L. Huergo +2 more
2024Frontiers in Microbiology6 citations Open access
Semantic Scholar
Multiplexed flow cytometric approach for detection of anti‐SARS‐CoV‐2 IgG, IgM and IgA using beads covalently coupled to the nucleocapsid protein
I. Zattoni, L. Huergo, E. M. Gerhardt, J. Nardin +5 more
2022Letters in Applied Microbiology6 citations Open access
Semantic Scholar
Controlling Protein Immobilization over Poly(3-hydroxybutyrate) Microparticles Using Substrate Binding Domain from PHA Depolymerase
Isabela P. Dias, R. D. da Cunha, R. Masaki, M. T. Todo Bom +14 more
2025Biomacromolecules4 citations Open access
Semantic Scholar
Magnetic bead-based ELISA allow inexpensive, rapid and quantitative detection of human antibodies against SARS-CoV-2
L. Huergo, M. Conzentino, E. M. Gerhardt, A. Santos +7 more
2020medRxiv4 citations Open access
Semantic Scholar
Biophysical characterization and molecular phylogeny of human KIN protein
J. R. Pattaro, Í. Caruso, Q. A. de Lima, F. F. Duarte +4 more
2019European Biophysics Journal4 citations
Semantic Scholar
A simple preparation of prestained molecular markers for electrophoresis using inexpensive and readily available proteins.
L. Chubatsu, E. M. Gerhardt, E. M. Souza
2023Analytical Biochemistry3 citations Open access
Semantic Scholar
A Sustainable Alternative for the Food Industry: Production of α-Amylase by Coprinus comatus Using Agro-Industrial By-Products
Luana Cristina Paludo, Bianca Peron-Schlosser, Rúbia Martins Bernardes Ramos, Pablo Inocêncio Monteiro +3 more
2025Processes2 citations Open access
Semantic Scholar
The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides
L. Huergo, M. Conzentino, M. V. Gonçalves, M. V. Gernet +11 more
2021World Journal of Microbiology & Biotechnology2 citations Open access
Semantic Scholar
Effect of Substrate-Binding Domain of Fusion Protein on Binding with Poly(3-hydroxybutyrate)-Based Nanocarrier
A. Sampaio, M. T. Todo Bom, M. L. Budel, Brenno Wendler Miranda +12 more
2025ACS Applied Nano Materials1 citations Open access
Semantic Scholar
Production and characterization of Laccase from Lentinus berteroi and applications for biodegradation of organic micropollutants
K. V. Avelino, Marisangela Isabel Wietzikoski Halabura, E. M. Gerhardt, M. Paetzold +3 more
2025Biodegradation1 citations
Semantic Scholar
Expression and purification of untagged GlnK proteins from actinobacteria
E. M. Gerhardt, V. Moure, Andrey W. Souza, F. O. Pedrosa +4 more
2017EXCLI Journal : Experimental and Clinical Sciences1 citations
Semantic Scholar
Metabolic regulation and structural mechanism of glutamine synthetase AMPylation
E. S. Lopes, Eijaz Ahmed Bhat, L. F. Tomazini, A. A. Stefanello +7 more
2026bioRxiv0 citations Open access
Semantic Scholar
Tailoring Fusion Proteins for Interfacial Nanoparticle Functionalization: Toward Fluorescence-Guided Imaging
R. Masaki, J. Lucena, M. T. Todo Bom, Isabela P. Dias +17 more
2026ACS Omega0 citations Open access
Semantic Scholar
Modulation of Glutamine Synthetase adenylylation by nitrogen availability enables one-step purification in distinct post-translational states.
L. F. Tomazini, E. S. Lopes, Barbara B. Nogueira, Gabriela Carvalho Calsavara +5 more
2025Protein Expression and Purification0 citations
Semantic Scholar
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense.
Erick Parize, E. M. Gerhardt, A. Oliveira, F. Pedrosa +3 more
2022Protein Expression and Purification0 citations
Semantic Scholar
Multiplexed Flow Cytometric Approach for Detection of Anti-SARS-CoV-2 IgG, IgM and IgA Using Beads Covalently Coupled to the Nucleocapsid Protein
Ingrid Fatima Zattoni, L. Huergo, E. M. Gerhardt, J. Nardin +5 more
20210 citations Open access
Semantic Scholar
Kinetics and structural features of dimeric glutamine-dependent bacterial NAD (cid:2) synthetases suggest evolutionary adaptation to available metabolites
A. Santos, E. M. Gerhardt, Vivian RotunoMoure, F. O. Pedrosa +4 more
20200 citations
Semantic Scholar
Magnetic bead-based ELISA allow inexpensive, rapid and quantitative detection of human antibodies SARS-CoV-2
L. Huergo, M. Conzentino, E. M. Gerhardt, Adrian Richard +9 more
20200 citations
Semantic Scholar
Biophysical characterization and molecular phylogeny of human KIN protein
J. R. Pattaro, Í. Caruso, Q. A. de Lima, F. F. Duarte +4 more
2019European Biophysics Journal0 citations
Semantic Scholar
Correction: Kinetics and structural features of dimeric glutamine-dependent bacterial NAD+ synthetases suggest evolutionary adaptation to available metabolites.
A. Santos, E. M. Gerhardt, V. Moure, F. O. Pedrosa +4 more
2019Journal of Biological Chemistry0 citations Open access
Semantic Scholar
In vitro interaction between the ammonium transport protein AmtB and partially uridylylated forms of the PII protein GlnZ Proteins and proteomics
Thiago E. Rodrigues, Victor E. P. Souza, R. Monteiro, E. M. Gerhardt +5 more
20110 citations
Semantic Scholar

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