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M. Hirata · SciRah

Researcher profile · Semantic Scholar

M. Hirata

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M. Hirata — researcher profile on SciRah

h-index: 17
Works: 96
Citations: 1,218

Most-cited papers

aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity.
A. Suzuki, M. Hirata, Katsusi Kamimura, R. Maniwa +8 more
2004Current Biology342 citations Open access
Semantic Scholar
Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation
F. Tanihara, M. Hirata, Nhien T. Nguyen, Q. A. Le +5 more
2018PLoS ONE58 citations Open access
Semantic Scholar
Journal of Reproduction and Development
F. Tanihara, M. Hirata, Shigeki Morikawa, Nhien T. Nguyen +5 more
201942 citations
Semantic Scholar
Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes
F. Tanihara, M. Hirata, Nhien T. Nguyen, Q. A. Le +4 more
2019Animal Biotechnology39 citations
Semantic Scholar
Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
F. Tanihara, M. Hirata, Nhien T. Nguyen, O. Sawamoto +3 more
2020BMC Biotechnology36 citations Open access
Semantic Scholar
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
Q. A. Le, F. Tanihara, M. Wittayarat, Z. Namula +5 more
2021BMC Research Notes35 citations Open access
Semantic Scholar
Effects of chlorogenic acid and caffeic acid on the quality of frozen‐thawed boar sperm
Z. Namula, M. Hirata, M. Wittayarat, F. Tanihara +4 more
2018Reproduction in domestic animals = Zuchthygiene35 citations Open access
Semantic Scholar
Effects of voltage strength during electroporation on the development and quality of in vitro‐produced porcine embryos
K. Nishio, F. Tanihara, T. V. Nguyen, Toshiki Kunihara +4 more
2018Reproduction in domestic animals = Zuchthygiene34 citations Open access
Semantic Scholar
Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes
Z. Namula, M. Wittayarat, M. Hirata, Takayuki Hirano +5 more
2019In vitro Cellular & Developmental Biology-Animal33 citations
Semantic Scholar
One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis
F. Tanihara, M. Hirata, Nhien T. Nguyen, O. Sawamoto +2 more
2021International Journal of Molecular Sciences32 citations Open access
Semantic Scholar
Generation of viable PDX1 gene‐edited founder pigs as providers of nonmosaics
F. Tanihara, M. Hirata, Nhien Thi Nguyen, Quynh Anh Le +2 more
2020Molecular Reproduction and Development31 citations
Semantic Scholar
Expression, purification, and inhibitory activities of mouse cytotoxic T-lymphocyte antigen-2α
Mitsuhiro Kurata, M. Hirata, S. Watabe, M. Miyake +2 more
200331 citations
Semantic Scholar
Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes
M. Hirata, F. Tanihara, M. Wittayarat, Takayuki Hirano +5 more
2019In vitro Cellular & Developmental Biology-Animal30 citations
Semantic Scholar
Current status of the application of gene editing in pigs
F. Tanihara, M. Hirata, T. Otoi
2021Journal of reproduction and development29 citations Open access
Semantic Scholar
Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.
F. Tanihara, M. Hirata, Nhien T. Nguyen, Q. A. Le +5 more
2018Animal science journal = Nihon chikusan Gakkaiho28 citations Open access
Semantic Scholar
Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes.
Q. A. Le, M. Hirata, Nhien T. Nguyen, Koki Takebayashi +6 more
2020Animal science journal = Nihon chikusan Gakkaiho21 citations
Semantic Scholar
Curcumin supplementation in the maturation medium improves the maturation, fertilisation and developmental competence of porcine oocytes.
Z. Namula, Yoko Sato, M. Wittayarat, Q. A. Le +5 more
2020Acta Veterinaria Hungarica18 citations Open access
Semantic Scholar
Generation of mutant pigs by lipofection-mediated genome editing in embryos
M. Hirata, M. Wittayarat, Z. Namula, Q. A. Le +7 more
2021Scientific Reports17 citations Open access
Semantic Scholar
One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs
M. Hirata, M. Wittayarat, F. Tanihara, Yoko Sato +5 more
2020In Vitro Cellular & Developmental Biology - Animal14 citations
Semantic Scholar
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation
M. Hirata, M. Wittayarat, Z. Namula, Q. A. Le +6 more
2020Molecular Biology Reports14 citations
Semantic Scholar
The Relationship between Embryonic Development and the Efficiency of Target Mutations in Porcine Endogenous Retroviruses (PERVs) Pol Genes in Porcine Embryos
M. Hirata, M. Wittayarat, Takayuki Hirano, Nhien T. Nguyen +5 more
2019Animals14 citations Open access
Semantic Scholar
Aberrant levels of DNA methylation and H3K9 acetylation in the testicular cells of crossbred cattle-yak showing infertility.
P. Phakdeedindan, M. Wittayarat, T. Tharasanit, M. Techakumphu +7 more
2021Reproduction in domestic animals = Zuchthygiene13 citations
Semantic Scholar
Gene editing in porcine embryos using a combination of electroporation and transfection methods.
Koki Takebayashi, M. Wittayarat, Qing-Yi Lin, M. Hirata +6 more
2022Reproduction in domestic animals = Zuchthygiene12 citations
Semantic Scholar
Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.
Z. Namula, F. Tanihara, M. Wittayarat, M. Hirata +5 more
2019Acta Veterinaria Hungarica12 citations Open access
Semantic Scholar
Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation
F. Tanihara, M. Hirata, Nhien T. Nguyen, Takayuki Hirano +2 more
2018Journal of Veterinary Medical Science12 citations Open access
Semantic Scholar
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.
T. Nguyen, M. Wittayarat, Lanh Thi Kim Do, T. V. Van Nguyen +6 more
2018Animal science journal = Nihon chikusan Gakkaiho12 citations Open access
Semantic Scholar
Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos
M. Hirata, M. Wittayarat, Z. Namula, Quynh Anh Le +5 more
2021Animals11 citations Open access
Semantic Scholar
Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.
T. V. Nguyen, F. Tanihara, M. Hirata, Takayuki Hirano +4 more
2018Cryo-letters11 citations
Semantic Scholar
Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
Qing-Yi Lin, Q. A. Le, Koki Takebayashi, C. Thongkittidilok +4 more
2021BMC Research Notes10 citations Open access
Semantic Scholar
Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.
Yoko Sato, Ryota Kuriwaki, Shiki Hagino, M. Shimazaki +6 more
2019Reproduction in domestic animals = Zuchthygiene10 citations
Semantic Scholar
Disruption of cell proliferation and apoptosis balance in the testes of crossbred cattle-yaks affects spermatogenic cell fate and sterility.
M. Shimazaki, M. Wittayarat, R. Sambuu, Asami Sugita +7 more
2022Reproduction in domestic animals = Zuchthygiene9 citations
Semantic Scholar
Effects of individual or in-combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos.
C. Thongkittidilok, Q. A. Le, Qing-Yi Lin, Koki Takebayashi +5 more
2021Reproduction in domestic animals = Zuchthygiene9 citations
Semantic Scholar
Mitochondrial-Targeted Protective Potential of Elamipretide for the In Vitro Production of Porcine Embryos
S. T. Nguyen, T. Otoi, Z. Namula, Oky Setio Widodo +7 more
2025Animals8 citations
Semantic Scholar
Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
F. Tanihara, M. Hirata, Z. Namula, Lanh Thi Kim Do +6 more
2023Frontiers in Cell and Developmental Biology8 citations Open access
Semantic Scholar
Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system
Z. Namula, M. Wittayarat, Lanh Thi Kim Do, T. V. Van Nguyen +5 more
2022Veterinary and Animal Science8 citations Open access
Semantic Scholar
Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides.
M. Wittayarat, M. Hirata, Z. Namula, Yoko Sato +6 more
2021Animal science journal = Nihon chikusan Gakkaiho8 citations
Semantic Scholar
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid
Nhien Thi Nguyen, M. Hirata, F. Tanihara, Takayuki Hirano +3 more
2019Reproduction in domestic animals = Zuchthygiene8 citations
Semantic Scholar
Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature.
Qing-Yi Lin, Q. A. Le, Koki Takebayashi, M. Hirata +5 more
2022Reproduction in domestic animals = Zuchthygiene7 citations
Semantic Scholar
Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection
Z. Namula, Q. A. Le, M. Wittayarat, Qing-Yi Lin +5 more
2022Veterinary World7 citations Open access
Semantic Scholar
Relationship among ovarian follicular status, developmental competence of oocytes, and anti-Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.
F. Tanihara, M. Hirata, S. Iizuka, Shin-ya Sairiki +5 more
2019Animal science journal = Nihon chikusan Gakkaiho7 citations Open access
Semantic Scholar
GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system
F. Tanihara, M. Hirata, Z. Namula, M. Wittayarat +6 more
2023Molecular Biology Reports6 citations
Semantic Scholar
GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines.
F. Tanihara, M. Hirata, T. Otoi
2023Methods in molecular biology6 citations
Semantic Scholar
Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes
Z. Namula, M. Hirata, Q. A. Le, Qing-Yi Lin +5 more
2021Veterinary Medicine and Science6 citations Open access
Semantic Scholar
Indole-3-propionic acid supplementation during in vitro maturation improves in vitro production of porcine embryos
Y. Nakayama, T. Otoi, Namula Zhao, S. T. Nguyen +9 more
2025BMC Veterinary Research5 citations Open access
Semantic Scholar
Comparison of chemically mediated CRISPR/Cas9 gene editing systems using different nonviral vectors in porcine embryos.
Qing-Yi Lin, Koki Takebayashi, N. Torigoe, Bin Liu +5 more
2023Animal science journal = Nihon chikusan Gakkaiho5 citations
Semantic Scholar
Short-term preservation of porcine zygotes at ambient temperature using a chemically defined medium.
Qing-Yi Lin, Q. A. Le, Koki Takebayashi, M. Hirata +4 more
2022Animal science journal = Nihon chikusan Gakkaiho5 citations
Semantic Scholar
Vaginal stimulation enhances ovulation of queen ovaries treated using a combination of eCG and hCG
Naoaki Yoshimura, M. Taniguchi, T. Terazono, Tetsushi Ono +5 more
2020Veterinary Medicine and Science5 citations Open access
Semantic Scholar
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts
F. Tanihara, M. Hirata, Shigeki Morikawa, Nhien T. Nguyen +5 more
2019Journal of reproduction and development5 citations Open access
Semantic Scholar
Expression, purification, and inhibitory activities of mouse cytotoxic T-lymphocyte antigen-2alpha.
Mitsuhiro Kurata, M. Hirata, S. Watabe, M. Miyake +2 more
2003Protein Expression and Purification5 citations
Semantic Scholar
Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes
Qing-Yi Lin, Koki Takebayashi, N. Torigoe, Bin Liu +5 more
2024In Vitro Cellular & Developmental Biology - Animal4 citations
Semantic Scholar

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