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[Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)].
M E Floccaria b, G Tiscornia, F Palmada, Méndez Bs
- aDepartamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
- bDepartamento de Química Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
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Abstract
Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.