article
Expression of Escherichia coli DcuS-R two-component regulatory system is regulated by the secondary internal promoter which is activated by CRP-cAMP.
Tomoya Oyamadaa, Katsushi Yokoyama, Michiko Morinaga, Masashi Suzuki, Kozo Makinob
- aDepartment of Applied Chemistry, National Defense Academy of Japan, Yokosuka, Japan
- bNational Defense Academy of Japan
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Abstract
The DcuS-R two-component system of Escherichia coli senses C4-dicarboxylates of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.