[Chiral separation of drugs based on macrocyclic antibiotics using HPLC, supercritical fluid chromatography (SFC) and capillary electrochromatography (CEC)].
J. Dungelováa, Jozef Lehotay, T Rojkovicová, Jozef Cizmárik
- aKatedra analytickej chémie Fakulty chemickej a potravinárskej technológie Slovenskej technickej univerzity, Bratislava
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Abstract
Separation of enantiomers by means of high-performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), and capillary electrochromatography (CEC) is of great importance in pharmaceutical analyses. In recent years, separation of various types of racemates employs most frequently chiral stationary phases based on macrocyclic antibiotics. This class of chiral selectors includes vancomycin, teikoplanin, ristocetin A, teikoplanin without saccharide components, avoparcin, etc. The review paper describes the properties of selected antibiotics, the effect of chromatographic conditions on enantioselectivity (effect of the composition of the mobile phase, effect of pH of the mobile phase, effect of temperature), and the study of possible mechanisms of interaction, which play an important role in the separation of enantiomers. Examples of the use of macrocyclic antibiotics in the separation of various compounds by means of the HPLC, SFC, and CEC methods follow.
TL;DRThe properties of selected antibiotics, the effect of chromatographic conditions on enantioselectivity, and the study of possible mechanisms of interaction are described, which play an important role in the separation of enantiomers. (Semantic Scholar)