Role and mechanism of AMH in the regulation of Sertoli cells in mice
Zia Ur Rehmana, Tesfaye Workua, John S. Davisb c, Hira Sajjad Talpura, Dinesh Bhattaraia, Ishwari Kadariyaa, Guohua Huaa, Jing Caoa, Rahim Dada, Farmanullah Farmanullaha, Tarique Hussaind, Liguo Yanga e✉
- aKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China
- bOlson Center for Women's Health, Omaha VA Medical Center, Omaha, NE, USA
- cDepartments of Obstetrics and Gynecology and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA
- dLab of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese of Academy of Science, Changsha, Hunan, 410125, People's Republic of China
- eKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. Electronic address: yangliguo2006@foxmail.com
- ✉Corresponding author
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Abstract
Sertoli cells produce anti-Müllerian hormone (AMH), a glycoprotein belonging to the transforming growth factor-beta family. AMH mediates the regression of Müllerian ducts in the developing male fetus. However, the role of AMH in the regulation of primary Sertoli cells remains unclear. The present study was designed to investigate the effect of AMH on the viability and proliferation of Sertoli cells, with an additional focus on stem cell factor (SCF). Treatment of Sertoli cells with increasing concentrations of rh-AMH (0, 10, 50, 100, and 800ng/ml) for two days revealed that AMH, at high concentrations, increased apoptosis. These results were confirmed by a significant increase in Caspase-3 and Bax and a decrease in Bcl-2 protein and mRNA expression (P<0.01). Paradoxically, treatment with a low concentration of rh-AMH (10ng/ml), but not higher concentrations (50-800ng/ml), promoted Sertoli cell proliferation, which was verified by an increase in PCNA mRNA (P<0.05). Furthermore, only low concentrations of rh-AMH activated the non-canonical ERK signaling pathway. Similarly, low concentrations of rh-AMH (10-50ng/ml) significantly increased (P<0.05) SCF mRNA and SCF protein levels. These findings indicate that AMH differentially regulates the fate of Sertoli cells in vitro by promoting proliferation at low concentrations and apoptosis at high concentrations. In addition, AMH increased the expression of SCF, an important regulator of Sertoli cell development. Therefore, AMH may play a role in Sertoli cell development.
TL;DRFindings indicate that AMH differentially regulates the fate of Sertoli cells in vitro by promoting proliferation at low concentrations and apoptosis at high concentrations, and increased the expression of SCF, an important regulator of SERToli cell development. (Semantic Scholar)