Multi drug resistant Pseudomonas aeruginosa: Pathogen burden and associated antibiogram in a tertiary care hospital of Pakistan
Waheed Ullaha, Muhammad Qasimb, Hazır Rahmanb, Fazli Baric, Saadullah Khana, Zia Ur Rehmana, Zahid Khand, Tamara Dworecke, Noor Muhammada f✉
- aDepartment of Biotechnology and Genetic Engineering, Kohat University of Science & Technology, Kohat, Khyber Pakhtunkhwa, Pakistan
- bDepartment of Microbiology, Kohat University of Science & Technology, Kohat, Khyber Pakhtunkhwa, Pakistan
- cDepartment of Microbiology, Lady Reading Hospital, Peshawar, Pakistan
- dInstitute of Chemical Sciences, University of Peshawar, Pakistan
- eDepartment of Biology, RWTH, Aachen University, Germany
- fDepartment of Biotechnology and Genetic Engineering, Kohat University of Science & Technology, Kohat, Khyber Pakhtunkhwa, Pakistan. Electronic address: noormwazir@yahoo.com
- ✉Corresponding author
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Abstract
Pseudomonas aeruginosa is an important pathogen of both community and hospital acquired infections, and a major threat to public health for continuous emergence of multi-drug resistance. Current prevalence and pattern of multidrug resistance in the clinical isolates of P. aeruginosa is reported here. Samples were collected from September 2013 to January 2014 tertiary care hospital, Peshawar. Samples were subjected to phenotypic and molecular based detection of P. aeruginosa and were further processed for multidrug resistance pattern. Out of 3700 samples, 102 were identified as MDR P. aeruginosa. Prevalence of MDR isolates were found in pus (34.3%), wounds (28.4%), urine (19.6%), blood (14.7%) and sputum (2.9%) respectively. Isolates were more resistant to Sulphamethoxazole/Trimethoprim (98.04%), Amoxycillin/Clavulanic acid, Doxycycline and Chloramphenicol (95.1%) each, while least resistant to Imipenem (43.1%), Cefoperazone/Sulbactam (50.98%) and Amikacin (53.9%). Extensive MDR pattern was observed in P. aeruginosa was found as (n = 17, 16.6%) isolates were resistant to all four classes of antibiotics. Increased burden of MDR P. aeruginosa was documented in the study. Moreover, some isolates were even resistant to four classes of antibiotics. Findings of the study will be helpful to devise an appropriate antibiotic treatment strategy against MDR P. aeruginosa to cope the chances of evolving resistant pathogens.
TL;DRCurrent prevalence and pattern of multidrug resistance in the clinical isolates of P. aeruginosa is reported, and some isolates were even resistant to four classes of antibiotics. (Semantic Scholar)