Report of a fatal case of dengue infection with hepatitis: Demonstration of dengue antigens in hepatocytes and liver apoptosis
Anne Couvelarda b c, Philippe Marianneaua b c, Christophe Bedela b c, Marie‐Thérèse Droueta b c, François Vachona b c, Dominique Hénina b c, Vincent Deubela b c✉
- aService Central d'Anatomie et de Cytologie Pathologiques, Hôpital Bichat-Claude Bernard, Paris, France
- bService de Réanimation des Maladies Infectieuses, Hôpital Bichat-Claude Bernard, Paris, France
- cUnité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, Paris, France
- ✉Corresponding author
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Abstract
A fatal case of dengue (DEN) infection associated with a spleen rupture and with hepatitis is reported here. Microscopic studies showed numerous areas of spleen rupture with hematomas and revealed necrotic foci in liver samples obtained at autopsy. Although hepatitis was reported in several cases of DEN fever, the mechanism of liver injury remains poorly understood. In this case, immunohistochemistry showed that DEN viral antigens were mostly detected in hepatocytes surrounding the necrotic foci. By in situ detection of DNA fragmentation, apoptotic hepatocytes were found to be colocated with DEN virus-infected hepatocytes. These findings suggest that hepatocytes are the major sites of DEN virus replication in the liver and that DEN virus induces apoptosis of hepatocytes in vivo.
TL;DRFindings suggest that hepatocytes are the major sites of DEN virus replication in the liver and that DEN virus induces apoptosis of hepatocytes in vivo. (Semantic Scholar)