Comparison of Rapid Centrifugation Assay with Conventional Tissue Culture Method for Isolation of Dengue 2 Virus in C6/36-HT Cells
Rosmari Rodríguez Rochea b, Mayling Álvarezb, María G. Guzmánb, Luís Morierb, Gustavo Kouríb
- aDepartment of Virology, PAHO/WHO Collaborator Center for Viral Diseases, "Pedro Kourí" Tropical Medicine Institute, Havana, Cuba
- bDepartment of Virology, PAHO/WHO Collaborator Center for Viral Diseases, “Pedro Kourı́” Tropical Medicine Institute, Havana, Cuba
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Abstract
A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.
TL;DRThe results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens. (Semantic Scholar)