A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control
Julia W. Pridgeona b, James J. Becnelb, Gary G. Clarkc d, Kenneth J. Linthicumc d
- aCenter for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Dr., Gainesville, FL 32608, USA. julia.pridgeon@ars.usda.gov
- bThese authors contributed equally
- cCenter for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Dr., Gainesville, FL 32608
- dCenter for Medical, Agricultural, and Veterinary Entomology, USDA–ARS, 1600 SW 23rd Dr., Gainesville, FL 32608
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Abstract
Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC50 values of a representative group of compounds were determined using this high-throughput screening method and compared with LD50 values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.
TL;DRA high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in the laboratory to quickly screen large numbers of chemicals for activity against mosquitoes to identify effective compounds. (Semantic Scholar)