Segregation of random amplified DNA markers in F1 progeny of conifers
John E. Carlsona, Lomas K. Tulsierama b, Jeffrey C. Glaubitza b, Victor Luka b, Chris Kauffeldtc d, Robert Anthony Rutledgec d
- aThe Biotechnology Laboratory and the Faculty of Forestry at the University of British Columbia, #237-6174 University Blvd., V6T 1Z3, Vancouver, B.C., Canada
- bThe Biotechnology Laboratory and the Faculty of Forestry at the University of British Columbia, Vancouver, B.C., Canada
- cForestry Canada, Petawawa National Forestry Institute, K0J 1J0, Chalk River, Ontario, Canada
- dForestry Canada, Petawawa National Forestry Institute, Chalk River., Canada
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Abstract
The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ("RAPD" markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. The diallel study confirmed the appropriate inheritance of RAPD markers in the F1 generation. The value of these dominant RAPD markers for genetic linkage mapping in trees was established from both theoretical and applied perspectives.
TL;DRThe recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. (Semantic Scholar)