Studies on the Isolation of Cryptococcus Neoformans from Fruits and Vegetables: Untersuchungen zur Isolierung von Cryptococcus neoformans von Früchten und Gemüsen
Mahendra Singh Pala✉, B. S. Mehrotrab
- aVallabhbhai Patel Chest Institute, University of Delhi
- bDean, Faculty of Science, Kumaun University, Nainital
- ✉Corresponding author
Papers open inside SciRah from the original publisher or repository; SciRah never hosts them.
Abstract
Summary: The occurrence of Cryptococcus neoformans was studied in 254 vegetables and 186 fruit samples collected from different markets of Delhi during 1978 to 1980. Cryptococcus neoformans was isolated from five (1.1%) of the 440 fruits and vegetables material examined. The varieties of fruits and vegetables which yielded C. neoformans were apple, guava, papaya, carrot and potato. The isolates were tested for their pathogenicity in Swiss mice and were found pathogenic as confirmed by mycological and histopathological techniques. Laboratory experiments were conducted to study the suitability of three varieties of fruits and two types of vegetables to support the growth of C. neoformans. Luxurient growth of the yeast could be obtained after cultures were inoculated onto sliced and autoclaved vegetables and fruits. However, the unsterilized vegetables and fruits failed to support the growth of C. neoformans because of their easy contamination with rapidly growing molds and bacteria. As far as could be ascertained there seems to be no report on the isolation of C. neoformans from the Carrot (Daucus carota). the Guava (Psidium guajava) and the Papaya (Carica papaya). Zusammenfassung: Das Vorkommen von Cryptococcus neoformans wurde an 254 Proben von Gemüse und 186 Früchten, die von verschiedenen Märkten in Delhi stammten, während der Jahre 1978 bis 1980 untersucht C. neoformans konnte bei fünf der insgesamt 440 Proben (1,1%) nachgewiesen werden. C. neoformans wurde dabei auf einem Apfel, einer Guava‐Frucht, einer Papaya‐Frucht, einer Karotte und einer Kartoffel gefunden. Im Tierversuch an Mäusen war die Pathogenität der Stämme nachzuweisen. In vitro zeigte C. neoformans gutes Wachstum auf verschiedenen autoklavierten Früchten und Gemüseproben. Dagegen wurde das Wachstum auf unsterilisierten Proben durch Kontamination mit Schimmelpilzen und Bakterien verhindert. Die Autoren stellen fest, daß der Nachweis von C. neoformans auf Karotte, Guava‐Frucht und Papaya‐Frucht hiermit zum ersten Mal geführt wurde.
TL;DRThe occurrence of Cryptococcus neoformans was studied in 254 vegetables and 186 fruit samples collected from different markets of Delhi during 1978 to 1980 to study the suitability of three varieties of fruits and two types of vegetables to support the growth of C. neoforms. (Semantic Scholar)

