Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes
Wasun Chantratitaa b, Erik A. Henchalc, Chalobon Yoosooka b✉
- aDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand
- bDepartment of Microbiology Faculty of Science, Mahidol University Bangkok, Thailand
- cDepartment of Virus Diseases, Walter Reed Army Institute of Research, Washington D.C., U.S.A
- ✉Corresponding author
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Abstract
An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures with the ease of preparation of photobiotin-labelled double-stranded DNA.
TL;DRAn assay for rapid detection of herpes simplex virus in infected cells is described and provided an alternative method for earlier detection of virus in cell cultures with the ease of preparation of photobiotin-labelled double-stranded DNA. (Semantic Scholar)