Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population
Xuanyi Wanga b✉, Mohammed Ansaruzzamanc d, Raul Vaze, Catarina Mondlanee, Marcelino E. S. Lucasf, Lorenz von Seidleinb, Jacqueline L. Deenb, Julia S. Ampuerog, Mahesh K. Purib, Taesung Parkb h, GB Nairc d,
- aThe International Vaccine Institute, Seoul, Korea. xywang@ivi.int
- bThe International Vaccine Institute, Seoul, South Korea
- cICDDR,B: Centre for Health and Population Research, Dhaka, Bangladesh
- dICDDR, B: Centre for Health and Population Research, Dhaka, Bangladesh
- eCHAEM, Beira, Mozambique
- fMinistry of Health, Maputo, Mozambique
- gMédecins Sans Frontières, Geneva, Switzerland
- hDepartment of Statistics, Seoul National University, Seoul
- iNational Institute of Child Health and Human Development, Bethesda, Maryland, USA
- jNational Institute of Child Health and Human Development, Bethesda, USA
- kWorld Health Organization, Geneva, Switzerland
- l*World Health Organization, Geneva, Switzerland
- mInstitut Pasteur, Antananarivo, Madagascar
Papers open inside SciRah from the original publisher or repository; SciRah never hosts them.
Abstract
BACKGROUND: Early detection of cholera outbreaks is crucial for the implementation of the most appropriate control strategies. METHODS: The performance of an immunochromatographic dipstick test (Institute Pasteur, Paris, France) specific for Vibrio cholerae O1 was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season (January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture. RESULTS: The overall sensitivity and specificity of the rapid test compared to culture were 95% (95% confidence interval [CI]: 91%-99%) and 89% (95% CI: 86%-93%), respectively. After stratification by type of sample (rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%. CONCLUSION: This one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed.
TL;DRThis one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed. (Semantic Scholar)