Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin
Liancheng Liua, Zhigang Baia b, Xuemei Maa b, Tingting Wanga b, Yao Yanga b, Zhongtao Zhanga b
- aDepartment of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China
- bBeijing Key Laboratory of Cancer Invasion and Metastasis Research, National Clinical Research Center for Digestive Diseases, Beijing Friendship Hospital, Beijing 100050, P.R. China
Papers open inside SciRah from the original publisher or repository; SciRah never hosts them.
Abstract
The present study aimed to evaluate the role of taxol resistance gene 1 (Txr1) in the development of oxaliplatin (L-OHP) resistance in gastric cancer (GC). Using SGC-7901 cells as a model, Txr1 was exogenously expressed or knocked down using small interfering RNA. Quantitative polymerase chain reaction (qPCR) and western blotting were performed to establish whether the Txr1 gene is involved in chemoresistance, and cell proliferation was assessed using an MTS assay. To this end, the mRNA and protein levels of Txr1, thrombospondin-1 and excision repair cross-complementing 1 protein were measured using qPCR and western blotting, respectively. Txr1-knockdown significantly increased the sensitivity of the SGC-7901 cells to L-OHP, whereas Txr1 overexpression promoted the resistance of the SGC-7901 cells to L-OHP. Exogenous Txr1 expression in the SGC-7901 cells induced L-OHP resistance, and the siRNA knockdown of Txr1 sensitized the human GC cells to L-OHP. Txr1 is, therefore, likely to play a role in L-OHP resistance, acting via TSP1, and should be investigated as a potential therapeutic target in the treatment of GC.