In-vitro displacement interaction of atenolol and amlodipine on binding with bovine serum albumin when co-administered
Md Ashraful Alama, MA Awala, Mahbub Mostofaa, Md Kamrul Islamb c, Nusrat Subhand e
- aDepartment of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202
- bDepartment of Physiology, Bangladesh Agricultural University, Mymensingh 2202
- cDept. of Physiology, Bangladesh Agricultural University, Mymensingh-2202
- dPharmacy Discipline, Khulna University, Khulna 9208
- ePharmacy Discipline, Khulna University, Khulna-9208
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Abstract
The binding of atenolol (selective ?1-blocker) and amlodipine (calcium channel blocker) to bovine serum albumin (BSA) was studied by equilibrium dialysis method in order to have an insight into the binding chemistry of these two to BSA. Free atenolol concentration was increased due to addition of amlodipine which reduced the binding of the compounds to BSA. However, the free fraction was increased to a level as it was expected from direct competitive displacement while the free atenolol concentration was increased according to increasing the amlodipine concentration when only the BSA was present. The result obtained when the binding site was blocked by sufficient amount of amlodipine was that the increment of free concentration of atenolol was prominent. When no amlodipine was added the free concentration of atenolol was only 28% whereas this release was 93 % to 98.01% when amlodipine was added with increasing concentration.
TL;DRThe binding of atenolol and amlodipine to bovine serum albumin (BSA) was studied by equilibrium dialysis method in order to have an insight into the binding chemistry of these two to BSA. (Semantic Scholar)