Can l ‐ascorbic acid and trans‐resveratrol protect H a C a T cells from fine particulate matter toxicity?
Krystian T. Mokrzynskia b c, Olga I. Krzysztynska‐Kuletab d, Mateusz Wojtalab d, Dawid Wnuke f, Michał Sarnab d, Tadeusz Sarnab d✉
- aDepartment of Biophysics and Cancer Biology, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University Krakow Poland
- bDepartment of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University Krakow Poland
- cDepartment of Biophysics and Cancer Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
- dDepartment of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
- eDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University Krakow Poland
- fDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
- ✉Corresponding author
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Abstract
Abstract Continuous exposure of human skin to air pollution can result in a range of undesirable skin conditions. In our recent study, UV and visible light were found to increase cytotoxicity of fine particulate matter (PM2.5) against human keratinocytes. Since it is impossible to avoid exposure of human skin to PM2.5, effective strategies are needed to reduce their damaging effects. l‐ascorbic acid and resveratrol were tested as potential topical agents against pollution‐related skin impairment. Although these agents were previously found to ameliorate PM‐dependent damage, the effect of light and seasonal variation of particles were not previously studied. EPR spin‐trapping, DPPH assay, and singlet oxygen phosphorescence were used to determine the scavenging activities of the antioxidants. MTT, JC‐10 and iodometric assays were used to analyze the effect on PM2.5‐induced cytotoxicity, mitochondrial damage and oxidation of lipids. Live‐cell imaging was employed to examine wound‐healing properties of cells. Light‐induced, PM2.5‐mediated oxidative damage was examined by immunofluorescent staining. Both antioxidants effectively scavenged free radicals and singlet oxygen produced by PM2.5, reduced cell death and prevented oxidative damage to HaCaT cells. l‐ascorbic acid and resveratrol, especially when applied in combination, can protect HaCaT cells against the dark and light induced toxicity of PM2.5.
TL;DRBoth antioxidants effectively scavenged free radicals and singlet oxygen produced by PM2.5, reduced cell death and prevented oxidative damage to HaCaT cells, showing that l‐ascorbic acid and resveratrol can protect HaCaT cells against the dark and light induced toxicity of PM2.5. (Semantic Scholar)