Efficient Anticancer Effect on Choroidal Melanoma Cells Induced by Tanshinone IIA Photosensitization
Juan Lia b c d e, Diandian Wei, Jianfeng Wang, Ning Li, Yu-Chen Fana c, Na Li, Sijie Zhaoa c, Kun Lif g✉, Feng-Yuan Sun
- aDepartment of Ophthalmology First Affiliated Hospital of Bengbu Medical College Bengbu Anhui China
- bTianjin Key Laboratory of Retinal Functions and Diseases Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science Eye Institute and School of Optometry Tianjin Medical University Eye Hospital Tianjin China
- cDepartment of Ophthalmology, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China
- dThese authors contributed equally to this work
- eTianjin Key Laboratory of Retinal Functions and Diseases, Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China
- fInstitute of Biomedical and Health Science School of Life and Health Science Anhui Science and Technology University Fengyang Anhui China
- gInstitute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, Anhui, China
- ✉Corresponding author
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Abstract
Abstract Tanshinone IIA (TanIIA) has multiple biological functions and already been clinically used to treat many cardiovascular diseases. TanIIA is a photoactive molecule and can be excited by light to generate 3TanIIA*. Generation of 3TanIIA* by TanIIA photosensitization indicates that TanIIA may serve as a photosensitizer to bring photodynamic damage to organisms. Therefore, human choroidal melanoma MUM‐2B cell was chosen as a superficial tumor model and the photodynamic effect of TanIIA on tumor cells was evaluated in this study. The results showed that TanIIA photosensitization could generate singlet oxygen in noncellular system. MTT, clone formation and wound‐healing assays showed that the survival and migration of MUM‐2B cells could be efficiently inhibited by TanIIA photosensitization. And then, laser confocal microscope and flow cytometry were used to try to elucidate related mechanism. It was found that TanIIA could pass through cellular membrane and preferably accumulate in nucleus. TanIIA photosensitization could efficiently induce cell apoptosis and necrosis, increase intracellular ROS levels, decrease mitochondria membrane potential, and lead to cell cycle arrest in G2/M phase. Our findings indicate that TanIIA photosensitization can exert remarkable toxicity on choroidal melanoma cells.
TL;DRThe results showed that TanIIA photosensitization could generate singlet oxygen in noncellular system and can exert remarkable toxicity on choroidal melanoma cells. (Semantic Scholar)