The Effect of Laser Photobiomodulation on Periodontal Ligament Stem Cells
Leila Gholamia b, Gilda Parsamaneshc d, Shiva Shahabie f, Marzieh S. Jazaerie f, Kaveh Baghaeic d✉, Reza Fekrazadg
- aDepartment of Periodontics School of Dentistry Hamadan University of Medical Sciences Hamadan Iran
- bDepartment of Periodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
- cBasic and Molecular Epidemiology of Gastrointestinal Disorder Research Center Research Institute for Gastroenterology and Liver Diseases Shahid Beheshti University of Medical Science Tehran Iran
- dBasic and Molecular Epidemiology of Gastrointestinal Disorder Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Science, Tehran, Iran
- eDental Research Center Hamadan University of Medical Sciences Hamadan Iran
- fDental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
- gDepartment of Periodontology Dental Faculty – Radiation Sciences Research Center Laser Research Center in Medical Sciences AJA University of Medical Sciences Tehran Iran
- ✉Corresponding author
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Abstract
Abstract Photobiomodulation (PBM) is considered as a noninvasive procedure with the potential of inducing favorable changes in cellular behavior. In this study, we aimed to evaluate the effects of near‐infrared low‐intensity laser PBM on proliferation, viability and osteogenic differentiation of stem cells isolated from human periodontal ligament. A 940‐nm diode laser with an energy density of 4 J cm−2 in a 100‐mW continuous wave was used for irradiation in 3 sessions every 48h. Cell viability was measured 24, 48 and 72 h after irradiation. The effects of laser on mineralized tissue deposition were evaluated by using Alizarin red staining after dividing cells into three groups of nonosteogenic medium (C−), an osteogenic medium without laser (C+), and an osteogenic medium with laser irradiation (L+). Gene expression levels were also evaluated by real‐time PCR. Our results showed no significant difference between MTT levels of the study and control groups. After 14 and 21 days, both L+ and C+ groups showed an increase in mineralized tissue formation compared to the C− group. There was an increase in VEGF and BMP expressions compared to C−. In conclusion, the irradiation setting used in this study may be able to improve mineralized tissue deposition.