Establishment of Photoaging In Vitro by Repetitive UVA Irradiation: Induction of Characteristic Markers of Senescence and its Prevention by PAPLAL with Potent Catalase Activity
Satoshi Yoshimotoa b, Moemi Yoshidaa b, Hideya Andoa b, Masamitsu Ichihashic d e f g h✉
- aDepartment of Applied Chemistry and Biotechnology Okayama University of Science Okayama Japan
- bDepartment of Applied Chemistry and Biotechnology, Okayama University of Science, Okayama, Japan
- cAnti‐Aging Medical Research Center Graduate School of Life and Medical Sciences Doshisha University Kyoto Japan
- dArts Ginza Clinic Tokyo Japan
- eFaculty of Pharmaceutical Science Kobe Gakuin University Kobe Japan
- fAnti-Aging Medical Research Center, Graduate School of Life and Medical Sciences, Doshisha University, Kyoto, Japan
- gArts Ginza Clinic, Tokyo, Japan
- hFaculty of Pharmaceutical Science, Kobe Gakuin University, Kobe, Japan
- ✉Corresponding author
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Abstract
Abstract To understand a role ofUVAradiation in photoaging of the skin, we established a model of photoaging cells using cultured human dermal fibroblasts. Repeated low‐doseUVAradiation for 10 consecutive days induced senescence in fibroblasts, characterized with (1) increased level of senescence‐associatedβ‐galactosidase, (2) flattened large cell shape, (3) accumulation of reactive oxygen species, (4) yellowish coloration and (5) expression of p16. These were also observed in chronologically aged fibroblasts (doubling times >20), whereas none of these were detected in young cells (doubling times <10). Collectively, we propose that fibroblasts exposed to repetitiveUVAradiation may be a good model of aged cells to study the mechanism of aging and photoaging and further to search for novel agents preventing cellular senescence. In addition, H2O2was produced in the culture medium by a single low dose ofUVAirradiation. Further,PAPLAL, a nanoparticle of platinum and palladium having potent catalase‐like activity, significantly delayed the onset of H2O2‐induced cell senescence. The present study strongly indicates that repetitive short‐termUVAirradiation induces aging of cells possibly via H2O2and may be suppressed by potent anti‐H2O2agents.
TL;DRIt is proposed that fibroblasts exposed to repetitive UVA radiation may be a good model of aged cells to study the mechanism of aging and photoaging and further to search for novel agents preventing cellular senescence. (Semantic Scholar)