NAD (P)H and Flavin Autofluorescence Correlation with ATP in Rat Livers with Different Metabolic Steady‐State Conditions
Anna Cleta Crocea b c d e f✉, Andrea Ferrignoc f, Laura Giuseppina Di Pasquac f, Clarissa Berardoc f, Giovanni F. Bottirolia b c d e f, Mariapia Vairettic f
- aDepartment of Biology and Biotechnology University of Pavia Pavia Italy
- bInstitute of Molecular Genetics Italian National Research Council (CNR) Pavia Italy
- cInternal Medicine and Therapy (IMT) University of Pavia Pavia Italy
- dDepartment of Biology and Biotechnology, University of Pavia, Pavia, Italy
- eInstitute of Molecular Genetics, Italian National Research Council (CNR), Pavia, Italy
- fInternal Medicine and Therapy (IMT), University of Pavia, Pavia, Italy
- ✉Corresponding author
Papers open inside SciRah from the original publisher or repository; SciRah never hosts them.
Abstract
The monitoring of NAD(P)H and flavin autofluorescence (AF) is at the basis of numerous investigations on energy metabolism. Nevertheless, the ability of these AF biomarkers to accurately represent the energy currency, ATP, is poorly explored. Here, we focused on the AF/ATP correlation in lean and fatty livers with different steady-state metabolic conditions, achieved after organ isolation, preservation and recovery, in a likely dependence on both liver intrinsic metabolic features and externally induced perturbations. Within these eventual, various conditions, a significant correlation was detected between liver NAD(P)H and flavin AF, measured via fiber-optic probe, and biochemical ATP data, strengthening AF as biomarker of energy metabolism in steady-state conditions for wide-ranging experimental and diagnostic applications.
TL;DRA significant correlation was detected between liver NAD(P)H and flavin AF, measured via fiber‐optic probe, and biochemical ATP data, strengthening AF as biomarker of energy metabolism in steady‐state conditions for wide‐ranging experimental and diagnostic applications. (Semantic Scholar)