Seasonal variation of colchicine content in Colchicum brachyphyllum and Colchicum tunicatum (Colchicaceae)
Feras Qasem Alalia b✉, Abdullah El‐Alalic, Khaled Tawahad e, Tamam El‐Elimata b
- aa Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy , Jordan University of Science and Technology , PO Box 3030, Irbid 22110 , Jordan
- bDepartment of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy , Jordan University of Science and Technology , PO Box 3030, Irbid 22110 , Jordan
- cb Department of Chemistry , Mu’tah University , AlKarak , Jordan
- dc Department of Pharmaceutical Sciences, Faculty of Pharmacy , University of Jordan , Amman 11942 , Jordan
- eDepartment of Pharmaceutical Sciences, Faculty of Pharmacy , University of Jordan , Amman 11942 , Jordan
- ✉Corresponding author
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Abstract
As part of our continuing investigation of Jordanian Colchicum species, (-)-colchicine content in C. brachyphyllum Boiss. & Haussk. ex Boiss and Colchicum tunicatum Feinbr (Colchicaceae), growing wild in Jordan, was determined during different growth stages. Using external reference standard, a reverse-phase gradient photo-diode array high performance liquid chromatography (PDA-HPLC) method was adapted. Underground parts in both species and during different growth stages, always showed higher (-)-colchicine content than the above ground parts. In C. brachyphyllum total (-)-colchicine content of underground parts during flowering stage was found to be about 0.15% (wt/wt), while that of aerial parts was only about 0.04% (wt/wt). In C. tunicatum total (-)-colchicine content of underground parts was found to be 0.12% (wt/wt), and 0.13% (wt/wt) during flowering and vegetating stages, respectively, while that of aerial parts was only about 0.04% (wt/wt) and 0.02% (wt/wt), respectively. In C. tunicatum, stems, roots and unripe seeds are the main storages of (-)-colchicine at flowering, vegetating and seeding stages, respectively, while in C. brachyphyllum, corms are the main storage of (-)-colchicine at flowering and seeding stages.
TL;DRIn C. tunicatum, stems, roots and unripe seeds are the main storages of (−)-colchicine at flowering, vegetating and seeding stages, respectively, while in C. brachyphyllum, corms are themain storage of (−-colchichine at Flowering and Seeding stages. (Semantic Scholar)