Facile and Unplugged Surface Plasmon Resonance Biosensor with NIR-Emitting Perovskite Nanocomposites for Fast Detection of SARS-CoV-2

Facile and Unplugged Surface Plasmon Resonance Biosensor with NIR-Emitting Perovskite Nanocomposites for Fast Detection of SARS-CoV-2 · SciRah

^aDepartment of Electro-Optical Engineering, National Taipei University of Technology, Taipei 10608, Taiwan
^bOptical Sciences Center, National Central University, Taoyuan 32001, Taiwan
^cThin Film Technology Center, National Central University, Taoyuan 32001, Taiwan
^dDepartment of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan
^eDepartment of Electronic Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan
^fDepartment of Mechanical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan
^gTeraOptics Corporation, Taoyuan 32472, Taiwan
^hCenter for Tropical Medicine and Infectious Disease Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
ⁱDepartment of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
^jClinical Microbiology Laboratory, Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
^kFaculty of Internal Medicine and Hepatitis Research Center, College of Medicine, and Center for Cohort Study, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
^lHepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
^mPh.D. Program in Translational Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, and Academia Sinica, Kaohsiung 80708, Taiwan
ⁿDivision of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
^oSchool of Medicine, College of Medicine, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan
^pDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
^qDepartment of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
^rGeneral Education Center, Ming Chi University of Technology, New Taipei City 24301, Taiwan
^sOrganic Electronics Research Center, Ming Chi University of Technology, New Taipei City 24301, Taiwan
✉Corresponding author

Abstract

The emergence of the coronavirus disease 2019 (COVID-19) pandemic prompted researchers to develop portable biosensing platforms, anticipating to detect the analyte in a label-free, direct, and simple manner, for deploying on site to prevent the spread of the infectious disease. Herein, we developed a facile wavelength-based SPR sensor built with the aid of a 3D printing technology and synthesized air-stable NIR-emitting perovskite nanocomposites as the light source. The simple synthesis processes for the perovskite quantum dots enabled low-cost and large-area production and good emission stability. The integration of the two technologies enabled the proposed SPR sensor to exhibit the characteristics of lightweight, compactness, and being without a plug, just fitting the requirements of on-site detection. Experimentally, the detection limit of the proposed NIR SPR biosensor for refractive index change reached the 10 –6 RIU level, comparable with that of state-of-the-art portable SPR sensors. In addition, the bio-applicability of the platform was validated by incorporating a homemade high-affinity polyclonal antibody toward the SARS-CoV-2 spike protein. The results demonstrated that the proposed system was capable of discriminating between clinical swab samples collected from COVID-19 patients and healthy subjects because the used polyclonal antibody exhibited high specificity against SARS-CoV-2. Most importantly, the whole measurement process not only took less than 15 min but also needed no complex procedures or multiple reagents. We believe that the findings disclosed in this work can open an avenue in the field of on-site detection for highly pathogenic viruses.

TL;DRA facile wavelength-based SPR sensor built with the aid of a 3D printing technology and synthesized air-stable NIR-emitting perovskite nanocomposites as the light source and capable of discriminating between clinical swab samples collected from COVID-19 patients and healthy subjects is developed. (Semantic Scholar)

Facile and Unplugged Surface Plasmon Resonance Biosensor with NIR-Emitting Perovskite Nanocomposites for Fast Detection of SARS-CoV-2

Abstract

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