Protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data
Yujia Jianga b, Yixin Lia c, Mengnan Chenga d, Jiangshan Xua d, Xiaoyu Weia d e✉, Chuanyu Liua d f g✉
- aBGI Research, Hangzhou 310030, China
- bDepartment of Biology, University of Copenhagen, 2100 Copenhagen, Denmark
- cCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- dBGI Research, Shenzhen 518083, China
- eBGI Research, Shenzhen 518083, China. Electronic address: weixiaoyu@genomics.cn
- fShanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan, China. Electronic address: liuchuanyu@genomics.cn
- gShanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan, China
- ✉Corresponding author
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Abstract
Spatial transcriptomics analysis allows the examination of the biological characteristics and spatial distribution of individual lung cells at a single-cell resolution. However, due to the presence of cavities in the alveoli of the lungs, it is challenging to section them for spatial transcriptomics experiments. Here, we present a protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data. We describe steps for lung perfusion, acquiring frozen slices, collecting cDNA from lung sections, and data analysis. For complete details on the use and execution of this protocol, please refer to Jiang et al.1
TL;DRA protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data is presented and steps for lung perfusion, acquiring frozen slices, collecting cDNA from lung sections, and data analysis are described. (Semantic Scholar)