C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells
Bruno G. Douradinhaa b c✉, Sean P. McBurneyc, Klécia Marília Soares de Melo Cassemiroc d, Amanda Pfaff Smithc, Neel K. Krishnae, Simon M. Barratt‐Boyesc f, Jared D. Evansc g, Eduardo J. M. Nascimentoc f, Ernesto T. A. Marquesc d f
- aFondazione Ri.MED, Via Bandiera 11, 90133 Palermo, Italy
- bUniversity of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA. Electronic address: brd49@cvr.pitt.edu
- cUniversity of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA
- dVirology and Experimental Therapy Laboratory, Aggeu Magalhães Research Center, Fiocruz, Recife, Pernambuco 50670-420, Brazil
- eDepartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Lewis Hall, Room 3184, 700W. Olney Road, Norfolk, VA 23507, USA
- fDepartment of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, 130 DeSoto Street, Pittsburgh, PA 15261, USA
- gDepartment of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, 523 Bridgeside Point II, 450 Technology Drive, Pittsburgh, PA 15219, USA
- ✉Corresponding author
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Abstract
Summary Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to Dengue Virus Envelope protein and to whole Dengue Virus serotype 2. Incubation of Dengue Virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.
TL;DRScreening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to Dengue Virus Envelope protein and to whole Dengue Virus serotype 2, suggesting reduced inflammatory responses. (Semantic Scholar)