Evaluation of serological markers to monitor the disease status of Indian post kala-azar dermal leishmaniasis
Debanjan Mukhopadhyaya b, Nilay Kanti Dasc, Sritama De Sarkarb, Alak Mannab, Dwijendra Nath Gangulyd, Joyashree Nath Barbhuiyac, Arup K. Maitrab, Avijit Hazrab, Mitali Chatterjeeb
- aDept. of Pharmacology, Institute of Postgraduate Medical Education and Research, 244 B, Acharya J C Bose Road, Kolkata 700 020, India
- bDepartment of Pharmacology, Institute of Postgraduate Medical Education & Research, 244 B Acharya J. C. Bose Road, Kolkata 700 020, India
- cDept. of Dermatology, Calcutta Medical College, Kolkata-700 073, India
- dDept. of Dermatology, School of Tropical Medicine, Kolkata-700 073, India
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Abstract
Post kala-azar dermal leishmaniasis (PKDL), a dermal sequel of visceral leishmaniasis presents with macular or polymorphic lesions. As immunological variations between these two forms have not been delineated, we evaluated levels of antileishmanial total Ig, IgG and its subclasses, IgM, IgE, IgG avidity, cytokines IL-10, IL-4, IL-13 and expression of CD19. The levels of Ig and IgG in polymorphic PKDL were higher than macular PKDL, while significant curtailment in levels of Ig, IgM and IgG following treatment was evident only in polymorphic PKDL. With regard to IgG subclasses, IgG1 and IgG3 were significantly raised in polymorphic PKDL, whereas in macular PKDL only IgG1 was elevated; treatment decreased levels of IgG1, IgG2 and IgG3 only in polymorphic PKDL; IgE levels were raised in both groups but no marked alterations occurred following treatment. The avidity of IgG was higher in polymorphic PKDL and correlated with duration of disease. IL-10 was higher in polymorphic PKDL and decreased significantly after treatment, whereas in macular PKDL IL-4 predominated. Taken together, in PKDL the humoral immune response was greater in the polymorphic variant than the macular form suggesting that serological markers may have a role in monitoring polymorphic PKDL.
TL;DRIn PKDL the humoral immune response was greater in the polymorphic variant than the macular form suggesting that serological markers may have a role in monitoring polymorphic PKDL. (Semantic Scholar)