Trypsin mediated one-pot reaction for the synthesis of red fluorescent gold nanoclusters: Sensing of multiple analytes (carbidopa, dopamine, Cu2+, Co2+ and Hg2+ ions)
Subhadeep Ghosha, Jigna R. Bhamorea, Naved I. Maleka, Z. V. P. Murthyb, Suresh Kumar Kailasaa c✉
- aDepartment of Applied Chemistry, S. V. National Institute of Technology, Surat 395 007, India
- bChemical Engineering Department, S. V. National Institute of Technology, Surat 395 007, India
- cDepartment of Applied Chemistry, S. V. National Institute of Technology, Surat 395 007, India. Electronic address: sureshkumarchem@gmail.com
- ✉Corresponding author
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Abstract
Herein, we fabricated fluorescent gold nanoclusters (Au NCs) by using trypsin as a ligand. The fabricated trypsin-Au NCs emit bright red color fluorescence upon the exposure of 365 nm UV light. The trypsin-Au NCs are stable and well dispersed in water, which exhibited strong red emission peak at 665 nm upon excitation wavelength of 520 nm. The red fluorescence of trypsin-Au NCs was greatly quenched by the addition of multiple analytes such as drugs (carbidopa and dopamine) and three divalent metal ions (Cu2+, Co2+ and Hg2+ ion). As a result, a novel fluorescence "turn-off" probe was developed for the detection of the above analytes with good selectivity and sensitivity. This method exhibits the detection limits for carbidopa, dopamine, Cu2+, Co2+ and Hg2+ ions are 6.5, 0.14, 5.2, 0.0078, and 0.005 nM, respectively. The trypsin-Au NCs were successfully applied to detect drugs (carbidopa, and dopamine) in pharmaceutical samples and metal ions (Cu2+, Co2+ and Hg2+ ion) in biofluids and water samples, exhibiting good precision and accuracy, which offers a facile analytical strategy for assaying of the above analytes in pharmaceutical and biological samples.
TL;DRThe trypsin-Au NCs were successfully applied to detect drugs in pharmaceutical samples and metal ions in biofluids and water samples, exhibiting good precision and accuracy, which offers a facile analytical strategy for assaying of the above analytes in pharmaceutical and biological samples. (Semantic Scholar)