An epidemiological survey of the current status of Zika and the immune interaction between dengue and Zika infection in Southern Taiwan
Wen‐Hung Wanga b, Aspiro Nayim Urbinaa c, Chia‐Ching Wud, C. C. Lina e, Arunee Thitithanyanontf, Wanchai Assavalapsakulg, Po‐Liang Lub e, Yen‐Hsu Chena b, Sheng‐Fan Wanga d e h✉
- aCenter for Tropical Medicine and Infectious Disease, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan
- bDivision of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan
- cProgram in Tropical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan
- dDepartment of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan
- eDepartment of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, 80708, Taiwan
- fDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand
- gDepartment of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand
- hDepartment of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan. Electronic address: wasf1234@kmu.edu.tw
- ✉Corresponding author
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Abstract
OBJECTIVES: This study was performed to examine the current status of Zika and the effects of pre-existing dengue immunity on Zika virus (ZIKV) infection in Southern Taiwan. METHODS: A phylogenetic tree was used to analyze the phylogeny of detected ZIKVs. Paired sera from dengue patients were collected for the determination of dengue and Zika infection. Plaque reduction neutralization tests (PRNT) and quantitative reverse transcription PCR (qRT-PCR) were used to determine the titers of neutralizing antibodies and viruses, respectively. An antibody-dependent enhancement (ADE) assay was used to evaluate the effect of anti-dengue antibodies on ZIKV infection. RESULTS: results for the 21 samples identified eight cases of primary dengue infection and 13 cases of secondary dengue infection; two samples were positive for ZIKV (MR766). Results from the ADE assay indicated that convalescent sera from primary and secondary dengue infection patients displayed significant ADE of the ZIKV infection when compared to healthy controls (p < 0.05). CONCLUSIONS: This study suggests that pre-existing dengue immunity facilitates ZIKV infection and that the continuous importation of ZIKV infection may pose a threat to indigenous Zika emergence in Southern Taiwan.
TL;DRIt is suggested that pre-existing dengue immunity facilitates ZikV infection and continuous importation of ZIKV infection may pose a threat to the indigenous Zika emergence in Southern Taiwan. (Semantic Scholar)