High-throughput proteomics and metabolomic studies guide re-engineering of metabolic pathways in eukaryotic microalgae: A review
Hillol Chakdara, Mafruha T. Hasanb, Sunil Pabbic, Helena K. M. Nevalainend e, Pratyoosh Shuklaf g h✉
- aICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Maunath Bhanjan, Uttar Pradesh 275103, India
- bSchool of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia
- cCentre for Conservation and Utilisation of Blue Green Algae (CCUBGA), Division of Microbiology, ICAR - Indian Agricultural Research Institute, New Delhi 110 012
- dDepartment of Molecular Sciences, Macquarie University, NSW 2109, Australia
- eBiomolecular Discovery and Design Research Centre, Macquarie University, Sydney, NSW 2109, Australia
- fEnzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India
- gSchool of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: pratyoosh.shukla@gmail.com
- hSchool of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India
- ✉Corresponding author
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Abstract
Eukaryotic microalgae are a rich source of commercially important metabolites including lipids, pigments, sugars, amino acids and enzymes. However, their inherent genetic potential is usually not enough to support high level production of metabolites of interest. In order to move on from the traditional approach of improving product yields by modification of the cultivation conditions, understanding the metabolic pathways leading to the synthesis of the bioproducts of interest is crucial. Identification of new targets for strain engineering has been greatly facilitated by the rapid development of high-throughput sequencing and spectroscopic techniques discussed in this review. Despite the availability of high throughput analytical tools, examples of gathering and application of proteomic and metabolomic data for metabolic engineering of microalgae are few and mainly limited to lipid production. The present review highlights the application of contemporary proteomic and metabolomic techniques in eukaryotic microalgae for redesigning pathways for enhanced production of algal metabolites.