A comparative study reveals the higher resolution of RAPD over ARDRA for analyzing diversity of Nostoc strains
Hillol Chakdara b, Sunil Pabbia c✉
- aCentre for Conservation and Utilisation of Blue Green Algae (CCUBGA), Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India
- bNational Bureau of Agriculturally Important Microorganisms (NBAIM), Kushmaur, Maunath Bhanjan, UP, 275103, India
- cCentre for Conservation and Utilisation of Blue Green Algae (CCUBGA), Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India. sunil.pabbi@gmail.com
- ✉Corresponding author
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Abstract
Nostoc is a diverse genus of filamentous cyanobacteria with tremendous potential for agricultural and industrial applications. Morphometric methods and routine 16S rDNA-based identification undermines the genetic diversity and impedes strain-level differentiation. A comparative study to deduce the discriminatory power of random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA restriction analysis (ARDRA) for analyzing the genetic diversity of 20 Nostoc strains of diverse geographical origin was carried out. The RAPD primer used in the study generated 100% polymorphic profile. HIP TG primer produced the highest number of bands and fragments. Five primers, viz. OPA 08, OPA 11, HIP GC, OPAH 02 and OPF 05 could produce unique bands for 11 strains. Cluster analysis using the RAPD profile showed 12.5-25% similarity among the strains. Following in silico restriction analysis, two restriction enzymes, viz. HaeIII and HinfI were selected for ARDRA. However, clustering based on the restriction pattern showed 22.5-100% similarity. Results of the present study clearly indicate higher resolution of RAPD which can be reliably used for strain-level differentiation of Nostoc strains.