Direct measurement of IGF-I and IGFBP-3 in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis
Laura Palaa b, Stefano Gianninib c, Elisabetta Rosid e, Barbara Crescib c, G. Scanob f, Subburaman Mohang h, Roberto Durantib f, Carlo Maria Rotellab c✉
- aDepartment of Physiopathology, University of Florence, Italy
- buniversity of Florence
- cDepartment of Physiopathology, Endocrinology Unit, Section of Metabolic Diseases, University of Florence, Viale Pieraccini 6, 50136, Florence, Italy
- dFondazione “Don C. Gnocchi” ONLUS, Pozzolatico, Florence, Italy
- eFondazione “Don C. Gnocchi” ONLUS, Pozzolatico
- fDepartment of Internal Medicine and Immunoallergology, Section of Respiratory Medicine, University of Florence, Italy
- gJerry Lewis Petty Memorial VA Medical Center, Loma Linda, CA, USA
- hJerry Lewis Petty Memorial VA Medical Center
- ✉Corresponding author
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Abstract
Idiopathic pulmonary fibrosis (IPF) is characterized by the rearrangement of extracellular matrix and progressive increase in the amount of fibrotic tissue in the lung. IGF-I is a potent profibrogenic molecule and its bioavailability is dependent on at least 6 binding proteins called IGFBPs. Among these, IGFBP-3 is the most represented in serum and in different connective tissues. The purpose of this study was to identify and characterize IGFBP-3 in bronchoalveolar lavage (BAL) fluids. We studied 11 patients with IPF and 6 normal subjects by performing baseline pulmonary function test and BAL. IGF-I and IGFBP-3 were measured by RIA in BAL and serum. No significant differences were observed between serum IGF-I and IGFBP-3 from IPF patients and normal subjects. Instead, the direct measurement in BAL revealed a significant increase of IGF-I and IGFBP-3 in IPF patients compared to normal subjects. BAL IGF-I and IGFBP-3 concentrations were significantly related to inspiratory vital capacity (IVC) and carbon dioxide partial pressure (PaCO2): the higher the value of IVC and the lower the value of PaCO2, the higher the level of IGF-I and IGFBP-3. In conclusion, IGFBP3 and IGF-I could be important local mediators of IPF. Their direct measurement in BAL in IPF patients could be used as a clinical marker of the disease, since high levels of IGFBP-3 and IGF-I in BAL are associated to the initial phase of the disease.
TL;DRIn conclusion, IGFBP3 and IGF-I could be important local mediators of IPF and their direct measurement in BAL in IPF patients could be used as a clinical marker of the disease, since high levels of IGFBP-3 andIGF-I in BAL are associated to the initial phase of the Disease. (Semantic Scholar)