Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12
Н. С. Ждановаa b, Natalia M. Astakhovab c, Sergey B. Kuznetsovb c, L. Schülerd, O. L. Serovb c
- aDevelopmental Genetics Laboratory, Russian Academy of Sciences, Siberian Department, Novosibirsk
- bDevelopmental Genetics Laboratory, Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Department, Novosibirsk, Russia
- cDevelopmental Genetics Laboratory, Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Department, 630090, Novosibirsk, Russia
- dInstitut für Tierzucht und Tierhaltung, Martin-Luther-Universitat Halle-Wittenberg, Halle, Germany
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Abstract
By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
TL;DRIt was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. (Semantic Scholar)