Natural compounds from Leea macrophylla enhance phagocytosis and promote osteoblasts differentiation by alkaline phosphatase, type 1 collagen, and osteocalcin gene expression
Sayema Raiyana b, Md. Atiar Rahmana b✉, Md Abdullah Al Mamunc d, Md. Muzammal Haque Asimc d, Arwa A. Makkie f, Dina Hajjare f, Walla Alelwanie f, Jitbanjong Tangpongg h, Bijo Mathewi j
- aDepartment of Biochemistry and Molecular Biology University of Chittagong Chittagong Bangladesh
- bDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh
- cDepartment of Genetic Engineering and Biotechnology Shahjalal University of Science and Technology Sylhet Bangladesh
- dDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh
- eDepartment of Biochemistry, College of Science University of Jeddah Jeddah Saudi Arabia
- fDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia
- gSchool of Allied Health Sciences Walailak University Nakhon Si Thammarat Thailand
- hSchool of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand
- iDepartment of Pharmaceutical Chemistry Ahalia School of Pharmacy Palakkad India
- jDepartment of Pharmaceutical Chemistry, Ahalia School of Pharmacy, Palakkad, India
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Abstract
The current study investigated the immunomodulating and osteoblast differentiation potential of the natural compounds from Leea macrophylla (LMN). Immunomodulatory effects have been investigated by the phagocytosis of Candida albicans using polymorphonuclear neutrophil cells in the in vitro slide method. A bioactivity-guided fractionation technique was used to evaluate the stimulating effect of L. macrophylla methanol extract on osteoblast differentiation using mouse osteoblastic cells. A low dose of LMN was found to stimulate the phagocytic effect better than a higher dose. The natural compounds from L. macrophylla have significantly increased alkaline phosphatase (ALP) and osteocalcin activities. The LMN promoted the osteoblast differentiation through upregulation of ALP, osteocalcin, and type 1 collagen in a dose-dependent manner. These natural compounds also upregulated ALP, osteocalcin, and type 1 collagen gene expressions. The data suggest that LMN has potential anabolic sequel on bone formation and osteoblast differentiation.
TL;DRThe data suggest that LMN has potential anabolic sequel on bone formation and osteoblast differentiation and that a low dose of LMN was found to stimulate the phagocytic effect better than a higher dose. (Semantic Scholar)